LotaV3, Main, Exploration, bibRecord, 000118

The cis-acting CTTC-P1BS module is indicative for gene function of LjVTI12, a Qb-SNARE protein gene that is required for arbuscule formation in Lotus japonicus.

Identifieur interne : 000118 ( Main/Exploration ); précédent : 000117; suivant : 000119

The cis-acting CTTC-P1BS module is indicative for gene function of LjVTI12, a Qb-SNARE protein gene that is required for arbuscule formation in Lotus japonicus.

Auteurs : Frédéric Lota [Allemagne] ; Sarah Wegmüller ; Benjamin Buer ; Shusei Sato ; Andrea Br Utigam ; Benjamin Hanf ; Marcel Bucher

Source :

The Plant journal : for cell and molecular biology [ 1365-313X ] ; 2013.

RBID : pubmed:23452278

Descripteurs français

KwdFr :
- Interférence par ARN (MeSH), Loteae (génétique), Loteae (métabolisme), Mycorhizes (physiologie), Protéines végétales (génétique), Protéines végétales (métabolisme), Solanum tuberosum (microbiologie), Solanum tuberosum (métabolisme).
MESH :
- génétique : Loteae, Protéines végétales.
- microbiologie : Solanum tuberosum.
- métabolisme : Loteae, Protéines végétales, Solanum tuberosum.
- physiologie : Mycorhizes.
- Interférence par ARN.

English descriptors

KwdEn :
- Lotus (genetics), Lotus (metabolism), Mycorrhizae (physiology), Plant Proteins (genetics), Plant Proteins (metabolism), RNA Interference (MeSH), Solanum tuberosum (metabolism), Solanum tuberosum (microbiology).
MESH :
- chemical , genetics : Plant Proteins.
- genetics : Lotus.
- metabolism : Lotus, Plant Proteins, Solanum tuberosum.
- microbiology : Solanum tuberosum.
- physiology : Mycorrhizae.
- RNA Interference.

Abstract

The majority of land plants live in symbiosis with arbuscular mycorrhizal fungi from the phylum Glomeromycota. This symbiosis improves acquisition of phosphorus (P) by the host plant in exchange for carbohydrates, especially under low-P availability. The symbiosome, constituted by root cortex cells accommodating arbuscular mycorrhizal fungal hyphae, is the site at which bi-directional exchange of nutrients and metabolites takes place. Uptake of orthophosphate (Pi) in the symbiosome is facilitated by mycorrhiza-specific plant Pi transporters. Modifications of the potato Pi transporter 3 (StPT3) promoter were analysed in transgenic mycorrhizal roots, and it was found that the CTTC cis-regulatory element is necessary and sufficient for a transcriptional response to fungal colonization under low-Pi conditions. Phylogenetic footprinting also revealed binary combination of the CTTC element with the Pi starvation response-associated PHR1-binding site (P1BS) in the promoters of several mycorrhiza-specific Pi transporter genes. Scanning of the Lotus japonicus genome for gene promoters containing both cis-regulatory elements revealed a strong over-representation of genes involved in transport processes. One of these, LjVTI12, encoding a member of the SNARE family of proteins involved in membrane transport, exhibited enhanced transcript levels in Lotus roots colonized with the arbuscular mycorrhizal fungus Glomus intraradices. Down-regulation of LjVTI12 by RNA interference resulted in a mycorrhiza-specific phenotype characterized by distorted arbuscule morphology. The results highlight cooperative cis-regulation which integrates mycorrhiza and Pi starvation signaling with vesicle trafficking in symbiosome development.

DOI: 10.1111/tpj.12120
PubMed: 23452278

Affiliations:

Le document en format XML

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<term>Plant Proteins (genetics)</term>
<term>Plant Proteins (metabolism)</term>
<term>RNA Interference (MeSH)</term>
<term>Solanum tuberosum (metabolism)</term>
<term>Solanum tuberosum (microbiology)</term>
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<front><div type="abstract" xml:lang="en">The majority of land plants live in symbiosis with arbuscular mycorrhizal fungi from the phylum Glomeromycota. This symbiosis improves acquisition of phosphorus (P) by the host plant in exchange for carbohydrates, especially under low-P availability. The symbiosome, constituted by root cortex cells accommodating arbuscular mycorrhizal fungal hyphae, is the site at which bi-directional exchange of nutrients and metabolites takes place. Uptake of orthophosphate (Pi) in the symbiosome is facilitated by mycorrhiza-specific plant Pi transporters. Modifications of the potato Pi transporter 3 (StPT3) promoter were analysed in transgenic mycorrhizal roots, and it was found that the CTTC cis-regulatory element is necessary and sufficient for a transcriptional response to fungal colonization under low-Pi conditions. Phylogenetic footprinting also revealed binary combination of the CTTC element with the Pi starvation response-associated PHR1-binding site (P1BS) in the promoters of several mycorrhiza-specific Pi transporter genes. Scanning of the Lotus japonicus genome for gene promoters containing both cis-regulatory elements revealed a strong over-representation of genes involved in transport processes. One of these, LjVTI12, encoding a member of the SNARE family of proteins involved in membrane transport, exhibited enhanced transcript levels in Lotus roots colonized with the arbuscular mycorrhizal fungus Glomus intraradices. Down-regulation of LjVTI12 by RNA interference resulted in a mycorrhiza-specific phenotype characterized by distorted arbuscule morphology. The results highlight cooperative cis-regulation which integrates mycorrhiza and Pi starvation signaling with vesicle trafficking in symbiosome development.</div>
</front>
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The cis-acting CTTC-P1BS module is indicative for gene function of LjVTI12, a Qb-SNARE protein gene that is required for arbuscule formation in Lotus japonicus.

The cis-acting CTTC-P1BS module is indicative for gene function of LjVTI12, a Qb-SNARE protein gene that is required for arbuscule formation in Lotus japonicus.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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